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primary antibody mouse anti sox 2  (R&D Systems)


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    R&D Systems primary antibody mouse anti sox 2
    Primary Antibody Mouse Anti Sox 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody mouse anti sox 2/product/R&D Systems
    Average 95 stars, based on 357 article reviews
    primary antibody mouse anti sox 2 - by Bioz Stars, 2026-02
    95/100 stars

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    primary antibody mouse anti sox 2  (R&D Systems)
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    Effect of TDE incubation in endogenous TdTomato and EGFP fluorescence in LysMCre +/− , mT/mG mouse heart samples and AlbCre +/− , mT/mG mouse liver samples. A, B, C, D) % of the original Signal-to-noise/background ratio values over 24h for TdTomato in samples incubated in PBS (control), TDE 60%, 80%, 90%, and 100% plus additional post- incubation PBS wash for A) TdTomato and B) EGFP in heart samples, C) TdTomato and D) EGFP in liver samples. N=3. Error bars shown as standard error. E) Representative images of TdTomato (left, red) and EGFP (right, green) visualization in liver samples after 24h incubation in PBS, 60% TDE 80% TDE, 90% TDE and TDE 100%. Confocal microscopy images, 10x magnification. F,G,H) Effect of TDE incubation in AlexaFluor 488 fluorescence. F) Signal-to- noise ratio values over time for AlexaFluor 488 in samples incubated in PBS (control) and TDE 97%. G,H) examples of DAPI (Blue) and AlexaFluor 488 (Green) visualization in samples incubated for 1h in B) PBS (control) and C) TDE 97%. 20x Confocal Microscopy images. Scale bar = 37.2µm. I,J,K) Results of DAPI (Blue) and <t>Sox2</t> (Green) IHC labelling in R1+H2O2+TDE- cleared mouse brain samples. 10x magnification confocal microscopy. I) DAPI, B) Sox2, K) channel merge. Bleaching: 45min 10% H2O2 in PBS, 65°C, glass container. R1+ TDE: 10 days R1 + 1-day TDE 97%, 37°C, shaker.
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    Effect of TDE incubation in endogenous TdTomato and EGFP fluorescence in LysMCre +/− , mT/mG mouse heart samples and AlbCre +/− , mT/mG mouse liver samples. A, B, C, D) % of the original Signal-to-noise/background ratio values over 24h for TdTomato in samples incubated in PBS (control), TDE 60%, 80%, 90%, and 100% plus additional post- incubation PBS wash for A) TdTomato and B) EGFP in heart samples, C) TdTomato and D) EGFP in liver samples. N=3. Error bars shown as standard error. E) Representative images of TdTomato (left, red) and EGFP (right, green) visualization in liver samples after 24h incubation in PBS, 60% TDE 80% TDE, 90% TDE and TDE 100%. Confocal microscopy images, 10x magnification. F,G,H) Effect of TDE incubation in AlexaFluor 488 fluorescence. F) Signal-to- noise ratio values over time for AlexaFluor 488 in samples incubated in PBS (control) and TDE 97%. G,H) examples of DAPI (Blue) and AlexaFluor 488 (Green) visualization in samples incubated for 1h in B) PBS (control) and C) TDE 97%. 20x Confocal Microscopy images. Scale bar = 37.2µm. I,J,K) Results of DAPI (Blue) and <t>Sox2</t> (Green) IHC labelling in R1+H2O2+TDE- cleared mouse brain samples. 10x magnification confocal microscopy. I) DAPI, B) Sox2, K) channel merge. Bleaching: 45min 10% H2O2 in PBS, 65°C, glass container. R1+ TDE: 10 days R1 + 1-day TDE 97%, 37°C, shaker.
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    Effect of TDE incubation in endogenous TdTomato and EGFP fluorescence in LysMCre +/− , mT/mG mouse heart samples and AlbCre +/− , mT/mG mouse liver samples. A, B, C, D) % of the original Signal-to-noise/background ratio values over 24h for TdTomato in samples incubated in PBS (control), TDE 60%, 80%, 90%, and 100% plus additional post- incubation PBS wash for A) TdTomato and B) EGFP in heart samples, C) TdTomato and D) EGFP in liver samples. N=3. Error bars shown as standard error. E) Representative images of TdTomato (left, red) and EGFP (right, green) visualization in liver samples after 24h incubation in PBS, 60% TDE 80% TDE, 90% TDE and TDE 100%. Confocal microscopy images, 10x magnification. F,G,H) Effect of TDE incubation in AlexaFluor 488 fluorescence. F) Signal-to- noise ratio values over time for AlexaFluor 488 in samples incubated in PBS (control) and TDE 97%. G,H) examples of DAPI (Blue) and AlexaFluor 488 (Green) visualization in samples incubated for 1h in B) PBS (control) and C) TDE 97%. 20x Confocal Microscopy images. Scale bar = 37.2µm. I,J,K) Results of DAPI (Blue) and Sox2 (Green) IHC labelling in R1+H2O2+TDE- cleared mouse brain samples. 10x magnification confocal microscopy. I) DAPI, B) Sox2, K) channel merge. Bleaching: 45min 10% H2O2 in PBS, 65°C, glass container. R1+ TDE: 10 days R1 + 1-day TDE 97%, 37°C, shaker.

    Journal: bioRxiv

    Article Title: White and Clearing: New Optical Tissue Clearing Method

    doi: 10.1101/2025.01.30.635653

    Figure Lengend Snippet: Effect of TDE incubation in endogenous TdTomato and EGFP fluorescence in LysMCre +/− , mT/mG mouse heart samples and AlbCre +/− , mT/mG mouse liver samples. A, B, C, D) % of the original Signal-to-noise/background ratio values over 24h for TdTomato in samples incubated in PBS (control), TDE 60%, 80%, 90%, and 100% plus additional post- incubation PBS wash for A) TdTomato and B) EGFP in heart samples, C) TdTomato and D) EGFP in liver samples. N=3. Error bars shown as standard error. E) Representative images of TdTomato (left, red) and EGFP (right, green) visualization in liver samples after 24h incubation in PBS, 60% TDE 80% TDE, 90% TDE and TDE 100%. Confocal microscopy images, 10x magnification. F,G,H) Effect of TDE incubation in AlexaFluor 488 fluorescence. F) Signal-to- noise ratio values over time for AlexaFluor 488 in samples incubated in PBS (control) and TDE 97%. G,H) examples of DAPI (Blue) and AlexaFluor 488 (Green) visualization in samples incubated for 1h in B) PBS (control) and C) TDE 97%. 20x Confocal Microscopy images. Scale bar = 37.2µm. I,J,K) Results of DAPI (Blue) and Sox2 (Green) IHC labelling in R1+H2O2+TDE- cleared mouse brain samples. 10x magnification confocal microscopy. I) DAPI, B) Sox2, K) channel merge. Bleaching: 45min 10% H2O2 in PBS, 65°C, glass container. R1+ TDE: 10 days R1 + 1-day TDE 97%, 37°C, shaker.

    Article Snippet: For the immunohistochemistry-immunofluorescence (IHC-IF) assays, thin-slice samples were incubated in 1 µg/mL mouse anti-Sox2 primary antibody (sc-365823, Santa Cruz Biotechnology, TX, USA) in a shaker at 4°C overnight.

    Techniques: Incubation, Fluorescence, Control, Confocal Microscopy